Not Released
Sample In stock
Experience: Works
Not Used
Get This Part
Device

Part:BBa_K771004:Design

Designed by: Guo Huaqing;Suo Yang   Group: iGEM12_SJTU-BioX-Shanghai   (2012-09-25)


Membrane Anchor 4 : ssDsbA-PDZ Domain-LGT-GBD Domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 115
    Illegal AgeI site found at 1174
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 345
    Illegal BsaI site found at 795


Design Notes

For both Membrane Accelerator and Membrane Switch


Source

Lgt and ssDsbA are obtained from E.coli genome; GBD domain and PDZ domain are obtained from Prof. John Dueber

References